CD58 Antikörper

Produktnummer ABIN118746

Dieses Anti-CD58-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD58 in FACS und WB. Geeignet für Human.

Kurzübersicht für CD58 Antikörper (ABIN118746)

Target: CD58

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD58 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB)

Klon: BRIC-5

Produktdetails anti-CD58 Antikörper

Spezifität: BRIC5 was produced in response to erythrocytes. It reacts by Immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.Aff. Const.:4 x 10e8 M-1

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Human erythrocytes.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD58

Target: CD58

Andere Bezeichnung: CD58 / LFA3

Hintergrund: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Synonyms: Ag3, LFA-3, Lymphocyte function-associated antigen 3

Gen-ID: 965

NCBI Accession: NP_001138294

UniProt: P19256